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US 7,928,285 B2Granted
Method of producing xenogenous antibodies using a bovine
AssigneeKyowa Hakko Kirin Co., Ltd.
InventorsJames M. Robl, Yoshimi Kuroiwa, Poothappillai Kasinathan, Isao Ishida, Kazuma Tomizuka
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Abstract
In general, the invention features genetically modified non-human mammals (e.g., bovines and other ungulates), and methods of making these mammals. In particular, the invention features transgenic ungulates having reduced levels of endogenous IgM heavy chain and/or prion protein.
Claims6
A method of producing xenogenous antibodies, said method comprising the steps of:
- (a) providing a transgenic bovine whose genome comprises a homozygous mutation of both alleles of two different unrearranged IgM heavy chain loci, said mutations disrupting IgM heavy chain production and said transgenic bovine producing less than 10% of endogenous IgM heavy chain relative to a control bovine lacking the mutations, wherein said bovine further comprises a nucleic acid encoding all or part of a xenogenous immunoglobulin locus that undergoes rearrangement and expresses a xenogenous immunoglobulin;
- (b) administering one or more antigens of interest to said bovine;
- and
- (c) recovering xenogenous antibodies from said bovine.
1 more element
Dependencies are inferred from the claim text.