Perhydrolase providing improved peracid stability
AssigneeE. I. Du Pont de Nemours and Company
InventorsRobert DiCosimo, John Edward Gavagan, Mark S. Payne
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Abstract
An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
Claims5
An isolated nucleic acid molecule selected from the group consisting of:
- (a) a polynucleotide encoding a polypeptide having perhydrolytic activity, said polypeptide comprising the amino acid sequence of SEQ ID NO:
- 10;
- (b) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO:
- 9;
- and
- (c) a polynucleotide fully complementary to the polynucleotide of (a) or (b).
Dependencies are inferred from the claim text.