Recombinant production of mixtures of antibodies
AssigneeMerus N.V.
InventorsPatrick H. C. Van Berkel, Ronald Hendrik Peter Brus, Ton Logtenberg, Abraham Bout
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Abstract
The invention provides methods for producing mixtures of antibodies from a single host cell clone, wherein, a nucleic acid sequence encoding a light chain and nucleic acid sequences encoding different heavy chains are expressed in a recombinant host cell. The recombinantly produced antibodies in the mixtures according to the invention suitably comprise identical light chains paired to different heavy chains capable of pairing to the light chain, thereby forming functional antigen-binding domains. Mixtures of the recombinantly produced antibodies are also provided by the invention. Such mixtures can be used in a variety of fields.
Claims23
A method of producing a host cell, said method comprising:
- introducing into a host cell at least one nucleic acid sequence encoding an immunoglobulin light chain and nucleic acid sequences encoding at least three different immunoglobulin heavy chains,
- wherein said at least three different immunoglobulin heavy chains are capable of pairing with said immunoglobulin light chain to form functional antigen binding domains of three or more non-identical antibodies.
A method of producing a host cell, said method comprising:
- providing a host cell comprising a nucleic acid sequence encoding an immunoglobulin light chain;
- introducing nucleic acid sequences encoding at least three different immunoglobulin heavy chains into said host cell,
- wherein said at least three different immunoglobulin heavy chains are capable of pairing with said immunoglobulin light chain to form functional antigen binding domains of the three or more non-identical antibodies.
Dependencies are inferred from the claim text.