# US 7,927,814 B2 · Antibody directed against gastrin-releasing peptide precursor and use thereof

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 7,927,814 B2
- Document ID: US7927814B2
- Status: Granted
- Kind: B2
- Application number: 11/974,820
- Filed: 2007-10-15
- Published: 2008-07-31
- Granted: 2011-04-19
- Earliest claimed: 2005-04-13
- Assignees: Advanced Life Science Institute, Inc.
- Inventors: Katsumi Aoyagi
- Web page: https://patentgrep.com/patent/US7927814B2

## CPC

- G01N 33/5752: of the lungs
- C07K 14/57572: {Gastrin releasing peptide (bombesin C07K7/086)}
- C07K 16/26: against hormones {; against hormone releasing or inhibiting factors}
- C07K 2317/34: Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
- G01N 2333/5758: Gastrin releasing peptide
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

Disclosed is a novel method for determination of ProGRP which is free from problems such as the dispersion in the values of measurements and operational constraints such as handling of a test sample. A method for the determination of a gastrin-releasing peptide precursor or a digest thereof using at least two different antibodies each of which can recognize a peptide comprising the partial amino acid sequence starting from amino acid residue 40 and ended at amino acid residue 75 in the amino acid sequence depicted in SEQ ID NO:1; and a method for the determination of a gastrin-releasing peptide precursor and/or a digest thereof using at least two different antibodies each of which can recognize a peptide comprising the partial amino acid sequence starting from amino acid residue 40 and ended at amino acid residue 79 in the amino acid sequence depicted in SEQ ID NO:1. The methods have such advantages that a detection sensitivity comparable to that in a conventional determination method can be achieved, that it is easy to handle a sample after the sample is collected, and that a highly reproducible measurements can be obtained.

## Claims

### Claim 1

A method for determining a gastrin-releasing peptide precursor or a digest thereof, comprising contacting a test sample with two or more different antibodies which recognize a peptide comprising a partial amino acid sequence consisting of amino acids 40 to 75 of the amino acid sequence shown in SEQ ID NO:2, wherein at least one antibody is a monoclonal antibody which is produced by hybridoma 3D6-2 deposited under Accession No. FERM BP-08669.

### Claim 2

A method for determining a gastrin-releasing peptide precursor or a digest thereof, comprising contacting a test sample with two or more different antibodies which recognize a peptide comprising a partial amino acid sequence consisting of amino acids 40 to 79 of the amino acid sequence shown in SEQ ID NO:2, wherein at least one antibody is a monoclonal antibody which is produced by hybridoma 3D6-2 deposited under Accession No. FERM BP-08669.

### Claim 3 (depends on 1, 2)

The method according to any one of claim 1 or 2, which is a sandwich immunoassay method.

### Claim 4 (depends on 1, 2)

The method according to any one of claim 1 or 2, wherein another of the two or more different antibodies is a monoclonal antibody which recognizes a peptide comprising the partial amino acid sequence consisting of amino acids 71 to 75 of the amino acid sequence shown in SEQ ID NO:2.

### Claim 5 (depends on 4)

The method according to claim 4, wherein the antibody which recognizes a peptide comprising the partial amino acid sequence consisting of amino acids 71 to 75 of the amino acid sequence shown in SEQ ID NO:2 is produced by hybridoma 2B10 deposited under Accession No. FERM BP-4110.