# US 7,927,588 B2 · Skin dressings containing oxidoreductase enzyme > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 7,927,588 B2 - Document ID: US7927588B2 - Status: Granted - Kind: B2 - Application number: 10/557,998 - Filed: 2004-06-04 - Published: 2006-12-07 - Granted: 2011-04-19 - Earliest claimed: 2003-06-09 - Assignees: Archimed LLP - Inventors: Paul James Davis; Andrew John Austin - Web page: https://patentgrep.com/patent/US7927588B2 ## CPC - C12N 9/0004: {Oxidoreductases (1.)} - A61L 15/38: containing enzymes - A61P 17/00: Drugs for dermatological disorders - A61P 17/02: for treating wounds, ulcers, burns, scars, keloids, or the like - A61P 43/00: Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 - C12N 9/96: Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS ## Abstract A skin dressing comprises a first dressing component (16) carrying oxidoreductase enzyme in dried condition; and a second dressing component (18) carrying a source of water, such that when the first and second dressing components are placed in fluid communication with each other, water migrates from the second component towards the first comportent and acts to hydrate enzyme carried by the first component, at least at the surface of the first component. The dressing components are kept separate before use, e.g. being sealed in separate sterile, water-impervious packages such as laminated aluminum foil pouches. In use of the dressing, the second dressing component is located on the skin of a human or animal, e.g. over a wound to be treated or on a region of skin to be treated for cosmetic or therapeutic purposes such as for treatment of acne or other skin conditions. The first dressing component is placed on top of the second component in fluid communication therewith. In embodiments comprising only first and second dressing components, the first dressing component is placed in direct contact with the second dressing component. Water from the second component migrates towards the first comportent and acts to hydrate enzyme carried by the first component, at least at points of contact at the interface between the first and second components. Once hydrated, the oxidoreductase enzyme can immediately begin functioning in known manner with beneficial effects. ## Claims ### Claim 1 A kit for forming a skin dressing, comprising: a first dressing component carrying immobilized oxidoreductase enzyme in dried condition; and a second dressing component carrying a source of water; wherein a substrate for the immobilized oxidoreductase enzyme is present in at least one of said first and second dressing components; wherein the second dressing component is for application to the skin and the first dressing component is for application above the second dressing component distal to said skin to form a skin dressing such that water migrates from the second dressing component towards the first dressing component and acts to hydrate the immobilized oxidoreductase enzyme in the first dressing component at least at the surface of the first dressing component proximate to the second dressing component, with the hydrated immobilized oxidoreductase enzyme catalyzing the conversion of atmospheric oxygen to hydrogen peroxide for transport through the first and second dressing components to the skin for conversion to oxygen, and wherein said first and second dressing components are provided separately from one another such that there is no fluid communication therebetween prior to combination on the skin to form a skin dressing. ### Claim 2 (depends on 1) The kit according to claim 1, wherein the oxidoreductase enzyme comprises glucose oxidase. ### Claim 3 (depends on 1) The kit according to claim 1, wherein the substrate is present in the second dressing component. ### Claim 4 (depends on 1) The kit according to claim 1, including a supply of iodide ions. ### Claim 5 (depends on 1) The kit according to claim 1, wherein the first dressing component and second dressing component are in the form of layers. ### Claim 6 (depends on 1) The kit according to claim 1, wherein the first dressing component comprises a support or carrier carrying the immobilized oxidoreductase enzyme. ### Claim 7 (depends on 6) The kit according to claim 6, wherein the support or carrier comprises dried hydrogel material. ### Claim 8 (depends on 7) The kit according to claim 7, wherein the hydrogel comprises hydrophilic polymer material. ### Claim 9 (depends on 8) The kit according to claim 8, wherein the polymer comprises poly(-2-acrylamido-2-methylpropane sulphonic acid) and/or salts thereof. ### Claim 10 (depends on 8, 9) The kit according to claim 8 or 9, wherein the hydrophilic polymer material is present at a concentration of at least 1% by weight based on the total weight of the gel. ### Claim 11 (depends on 1) The kit according to claim 1, wherein the second dressing component comprises a support or carrier carrying water. ### Claim 12 (depends on 11) The kit according to claim 11, wherein the second dressing component comprises a hydrated hydrogel. ### Claim 13 (depends on 12) The kit according to claim 12, wherein the hydrated hydrogel comprises poly(-2-acrylamido-2-methylpropane sulphonic acid) and/or salts thereof. ### Claim 14 (depends on 13) The kit according to claim 13, wherein the second dressing component comprises a hydrated hydrogel comprising 20% by weight of poly(-2-acrylamido-2-methylpropane sulphonic acid) and/or a salt or salts thereof. ### Claim 15 (depends on 12, 13, 14) The kit according to claim 12, 13 or 14, wherein the hydrated hydrogel contains at least 30% by weight water. ### Claim 16 (depends on 14) The kit according to claim 14, wherein the hydrated hydrogel contains about 60% by weight of water. ### Claim 17 (depends on 1) The kit according to claim 1, wherein the first and second dressing components both include gel supports comprising the same polymer. ### Claim 18 (depends on 1) The kit according to claim 1, wherein the first dressing component includes monomers that are identical to monomers from which a polymeric support of the second dressing component is formed. ### Claim 19 (depends on 1) The kit according to claim 1, wherein the first dressing component includes one or more hydration enhancers. ### Claim 20 (depends on 19) The kit according to claim 19, wherein the hydration enhancer is selected from dried sugars, glycerol and sorbitol. ### Claim 21 (depends on 1) The kit according to claim 1, wherein the first dressing component comprises a support of dried hydrogel formed from 10% by weight poly(-2-acrylamido-2-methylpropane sulphonic acid) and/or a salt or salts thereof, carrying enzyme, and the second dressing component comprises a support of hydrated hydrogel comprising 20% by weight of poly(-2-acrylamido-2-methylpropane sulphonic acid) and/or a salt or salts thereof. ### Claim 22 (depends on 1) The kit according to claim 1, wherein the first and/or second dressing component includes one or more moisturizer materials. ### Claim 23 (depends on 1) The kit according to claim 1, including a covering or outer layer for adhering the dressing to the skin of a human or animal subject. ### Claim 24 (depends on 1) The kit according to claim 1, wherein the first and second dressing components are in separate sealed packages. ### Claim 25 (depends on 10) The kit according to claim 10 wherein the concentration of the hydrophilic polymer material is at least 5% by weight based on the total weight of the gel. ### Claim 26 (depends on 10) The kit according to claim 10 wherein the concentration of the hydrophilic polymer material is at least 10% by weight based on the total weight of the gel. ### Claim 27 (depends on 20) The kit according to claim 20 wherein the dried sugar is selected from the group consisting of sucrose and trehalose.