Photocleavable linker methods and compositions
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsJames K. Chen, Surajit Sinha, Ilya Shestopalov, Xiaohu Ouyang
Life of this patent
Estimating the term…
Abstract
Bifunctional linkers are provided that comprise a photocleavable moiety flanked by two different amine reactive moieties. In some embodiments the photocleavable moiety is a dimethoxynitrobenzyl moiety. In other embodiments the photocleavable moiety is 8-bromo-7-hydroxyquinoline. In other embodiments the photocleavable moiety is nitrodibenzofuran. In other embodiments the photocleavable moiety is 6-bromo-7-hydroxycoumarin-4-ylmethyl. The linkers find use in synthetic methods, including the generation of photocleavable oligonucleotides, e.g. caged morpholinos.
Claims5
A bifunctional linker having the structure:
- where X is a photocleavable moiety;
- R1 and R2 are independently selected from succinimide (NHS ester):
- isothiocyanate:
- propargyl:
- iodoacetamide:
- maleimide:
- azide:
- and terminal alkene:
- R3, R4 and R5 are independently selected from H and C1-C6 lower alkyls;
- and
- n is an integer from 0 to 10.
Dependencies are inferred from the claim text.