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US 7,923,551 B2Granted
Method of purifying RNA using kosmotropic salt
AssigneeSamsung Electronics Co., Ltd.
InventorsMyo-yong Lee, Nam Huh, Joon-ho Kim
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Abstract
The present invention provides a method of purifying RNA, including contacting a solid support with an acidic solution having a RNA-containing sample and a kosmotropic salt having a concentration of less than 1M, thereby binding the RNA to the solid support. According to the present invention, RNA is purified efficiently due to high RNA yield and low contamination by DNA. The present invention is particularly effective in purifying RNAs of 200 nucleotides or less.
Claims17
A method of isolating RNA comprising the steps of:
- a) providing a sample comprising a mixture of RNA and DNA, wherein the sample is resuspended in an acidic binding solution comprising a kosmotropic salt at a concentration of less than or equal to about 1M;
- b) contacting the sample with a solid support, wherein the RNA selectively binds to the solid support;
- and
- c) isolating the RNA bound to the solid support.
1 more element
A method of isolating RNA comprising the steps of:
- a) providing a sample comprising a mixture of RNA and DNA, wherein the sample is resuspended in an acidic binding solution comprising a kosmotropic salt at a concentration of less than or equal to about 1M, wherein the pH of the acidic binding solution ranges from 3 to 6 and the kosmotropic salt does not comprise Mg2+;
- b) contacting the sample with a solid support, wherein the RNA selectively binds to the solid support;
- and
- c) isolating the RNA bound to the solid support.
1 more element
Dependencies are inferred from the claim text.