High-purity rebaudioside A and method of extracting same
AssigneeChengdu Wagott Pharmaceutical Co., Ltd.
InventorsMingfu Yang, Jun Hua, Ling Qin
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Abstract
A process for recovering Rebaudioside A from Stevia rebaudiana Bertoni plants is provided and includes the steps of sequentially extracting plant material with a first solvent to obtain a first extract, filtering the first extract and passing the first extract through a polar resin to obtain a filtrate, and purifying the filtrate to obtain a crystalline-containing material. With further purification, a product containing over 99% Rebaudioside A is obtained. The present invention is also directed toward a method of purifying stevia glycosides to obtain purified Rebaudioside A. In one embodiment, the method includes silica gel column chromatography using an solvent comprising ethyl acetate and ethanol.
Claims43
A method for producing purified Rebaudioside A, comprising the steps of:
- providing a quantity of Stevia rebaudiana Bertoni;
- producing a crude extract by contacting the quantity of Stevia rebaudiana Bertoni with a first solvent;
- filtering the crude extract to obtain a filtrate feed containing Rebaudioside A;
- adsorbing the Rebaudioside A onto a polar resin by passing the filtrate feed over the polar resin;
- desorbing the Rebaudioside A from the polar resin by eluting with a second solvent to obtain an eluent containing Rebaudioside A;
- forming a first concentrated eluent by concentrating the eluent containing Rebaudioside A to a concentration of at least about 65% Rebaudioside A on a dry weight basis;
- subjecting the first concentrated eluent to a crystallization stage to obtain at least about 85% Rebaudioside A on a dry weight basis;
- and
- isolating the Rebaudioside A as crystals or as a solution.
A method for producing purified Rebaudioside A, comprising the steps of:
- providing a quantity of stevia glycosides;
- separating the stevia glycosides by column chromatography to obtain a crude extract, wherein the column chromato aphy proceeds by eluting with an eluting solvent comprising an about 20:1 to 20:4 volume ratio of ethyl acetate and water-ethanol mixed solvent;
- contacting the crude extract with a solvent to form a mixture;
- and
- purifying the mixture to obtain Rebaudioside A crystals.
Dependencies are inferred from the claim text.