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US 7,923,533 B2Granted
Methods for arbitrary peptide synthesis
AssigneeThe Invention Science Fund I, LLC
InventorsRoderick A. Hyde, Edward K. Y. Jung, Lowell L. Wood, Jr.
Life of this patent
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Abstract
Methods, apparatus, systems, computer programs and computing devices related to biologically assembling and/or synthesizing peptides and/or proteins are disclosed.
Claims60
A method comprising:
- determining, with one or more computing units, an order that two or more types of charged tRNA are to be co-localized sequentially with one or more ribosome-based assemblers in a reaction chamber of a fluidic device;
- controlling the operation of the fluidic device with the one or more computing units so that the two or more types of charged tRNA are individually sequentially flowed through one or more channels in the fluidic device to the reaction chamber according to the determined order;
- and
- assembling a target peptide by co-localizing sequentially the one or more ribosome-based assemblers and the two or more types of charged tRNA in the reaction chamber.
1 more element
A method of extra-cellular peptide synthesis comprising:
- determining, with one or more computing units, an order that two or more types of charged tRNA are to be co-localized sequentially with one or more ribosome-based assemblers in a reaction chamber of a fluidic device;
- controlling the operation of the fluidic device with the one or more computing units so that the two or more types of charged tRNA are individually sequentially flowed through one or more channels in the fluidic device to the reaction chamber according to the determined order;
- and
- assembling a target peptide in vitro by co-localizing sequentially the one or more ribosome-based assemblers, and the two or more types of charged tRNA.
1 more element
A method comprising:
- determining an assembly order with one or more computing units;
- controlling the operation of a fluidic device with the one or more computing units so that the two or more types of charged tRNA are individually sequentially flowed through one or more channels in the fluidic device to a reaction chamber of the fluidic device according to the determined assembly order;
- and
- assembling a target peptide by co-localizing the one or more ribosome-based assemblers, and the two or more types of charged tRNA in the reaction chamber.
1 more element
A method comprising:
- determining, with one or more computing units, a timing that two or more types of charged tRNA are to be co-localized sequentially with one or more ribosome-based assemblers in a reaction chamber of a fluidic device;
- controlling the operation of the fluidic device with the one or more computing units so that the two or more types of charged tRNA are individually sequentially flowed through one or more channels in the fluidic device to the reaction chamber according to the determined timing;
- and
- assembling a target peptide by co-localizing sequentially the one or more ribosome-based assemblers and the two or more types of charged tRNA in the reaction chamber.
1 more element
Dependencies are inferred from the claim text.