Methods for producing microspore derived doubled haploid apiaceae
AssigneeNRC Corporation
InventorsAlison M. R. Ferrie, Marie L. Mykytyshyn, Terry Bethune
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Abstract
The present invention relates to culturing isolated microspores and the subsequent generation of doubled-haploid plant lines that are suitable for the rapid selection of plants with improved composition and agronomic performance. Processes developed for the recovery of microspore-derived embryos from fennel and caraway may be adapted to related species in the Apiaceae family including, but not limited to, fennel, carrot, dill, anise, lovage, parsnip, and laceflower.
Claims16
A method for producing a microspore-derived doubled haploid plant of the family Apiaceae, the method comprising:
- isolating microspores from flower buds of microspore donor plants, wherein the microspores are at a developmental stage competent for induction of embryo development;
- culturing the isolated microspores in a medium comprising NLN or AT-3;
- inducing embryogenesis in the isolated microspore cultures;
- recovering the microspore-derived embryos;
- and
- generating doubled haploid plants.
A method for producing a microspore-derived embryo of a member of the Apiaceae (Umbelliferae) family of plants, the method comprising:
- isolating microspores from flower buds of microspore donor plants;
- culturing the isolated microspores in a medium comprising NLN or AT-3;
- inducing embryogenesis in the isolated microspore cultures;
- and
- generating a microspore-derived embryo.
Dependencies are inferred from the claim text.