US 7,923,247 B2 · Method for in vitro proliferation of dendritic cell precursors and their use to produce immunogens

Abstract

A method for producing proliferating cultures of dendritic cell precursors is provided. Also provided is a method for producing mature dendritic cells in culture from the proliferating dendritic cell precursors. The cultures of mature dendritic cells provide an effective means of producing novel T cell dependent antigens comprised of dendritic cell modified antigens or dendritic cells pulsed with antigen, including particulates, which antigen is processed and expressed on the antigen-activated dendritic cell. The novel antigens of the invention may be used as immunogens for vaccines or for the treatment of disease. These antigens may also be used to treat autoimmune diseases such as juvenile diabetes and multiple sclerosis.

Claims11

Dependent claims

A method of producing a population of dendritic cells comprising the steps of:

(a) providing a tissue source comprising dendritic cell precursors;
(b) treating the tissue source to enrich the proportion of dendritic precursor cells;
(c) culturing cells obtained from treatment of the tissue source in medium comprising GM-CSF at a concentration of about 400-800 U/ml and IL-4 at a concentration of about 500-1000 U/ml for a period of time sufficient to produce dendritic cells.

Dependencies are inferred from the claim text.

Facts

Application

12/218,899Filed 17 Jul 2008 · continuation

Priority

1 Apr 1992

Published

US 2009/0029469 A129 Jan 2009

Granted

12 Apr 2011

Term ends

Not estimatedThe source records don’t support an estimate.

Classification15 codes

C12N 5/0639
Cells from the blood or the immune system: dendritic cells, e.g. Langherhans cells in the epidermis
A61K 39/0008
Vertebrate antigens: antigens related to auto-immune diseases; Preparations to induce self-tolerance
A61K 40/19
Characterised by the cell type used: dendritic cells
A61K 40/22
Characterised by the effect or the function of the cells: immunosuppressive or immunotolerising

Family

Application07/861,612Parent of this continuation-in-part · filed 1 Apr 1992

Application07/981,357Parent of this continuation-in-part · filed 25 Nov 1992

Application08/040,677Parent of this continuation-in-part · filed 31 Mar 1993

Application08/261,537Parent of this division · filed 17 Jun 1994

Application09/451,511Parent of this continuation · filed 30 Nov 1999

Application10/287,813Parent of this continuation · filed 5 Nov 2002

US 2009/0029469 A1Pre-grant publication · published 29 Jan 2009

US 6,475,483 B1Parent of this continuation

US 5,994,126 AParent of this division

Application09/451,511Parent of this division

Application08/261,537Parent of this continuation-in-part

US 2003/0134419 A1Published application

US 7,923,247 B2This patent

From the US filing history. Foreign family members aren’t listed.

Method for in vitro proliferation of dendritic cell precursors and their use to produce immunogens

Life of this patent

Method for in vitro proliferation of dendritic cell precursors and their use to produce immunogens

Life of this patent

Abstract

Claims11