Artificial cartilage tissue and production method thereof
AssigneePG Research Co., Ltd.
InventorsSatoshi Miyauchi, Tatsuya Miyazaki, Satoshi Matsuzaka
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Abstract
To obtain an artificial cartilage tissue having high strength and biocompatibility. There is provided an artificial cartilage tissue which is substantially free of heterologous protein and is substantially free of scaffold derived from non-cartilage-derived cells, but contains mammalian cartilage-derived cells and an extracellular matrix formed by the cartilage-derived cells, where: (1) the water retention per mg of tissue dry weight is 17.8 mg or less; (2) collagen of 5.57 μg or more per mg of tissue wet weight is contained; (3) proteoglycan of 9.22 μg or more per mg of tissue wet weight is contained.
Claims8
An artificial cartilage tissue to which no bovine serum or plasma has been added and which does not contain an exogenous scaffold derived from non-cartilage-derived cells, but contains mammalian cartilage-derived cells and an extracellular matrix formed by the cartilage-derived cells, and has at least one of the following properties (1), (2), and (3):
- (1) a water retention per mg of tissue dry weight of 17.8 mg or less;
- (2) collagen of 5.57 μg or more per mg of tissue wet weight is contained;
- and
- (3) proteoglycan of 9.22 μg or more per mg of tissue wet weight is contained, wherein the mammalian cartilage-derived cells are only those obtained by culturing under the presence of serum or plasma derived from a mammal of the same species, wherein,
- the artificial cartilage tissue is obtained by culturing in a medium containing a growth factor belonging to the transforming growth factor-β superfamily.
Dependencies are inferred from the claim text.