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US 7,923,243 B2Granted
Stress-responsive induction of a therapeutic agent and methods of use
AssigneeUniversity of Southern California
InventorAmy S. Lee
Life of this patent
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Abstract
This invention relates to compositions and methods for selective expression of a heterologous nucleic acid sequence in a targeted tissue, and more particularly to the glucose regulated protein 78 (grp78) stress-responsive promoter and its use in gene therapy and the production of transgenic animals.
Claims9
A recombinant retroviral vector comprising a nucleic acid construct, the nucleic acid construct comprising:
- a) at least one glucose responsive protein 78 (grp78) non-coding regulatory sequence comprises a sequence from about 520 base pairs 5′ of the site of initiation of transcription of the rat glucose responsive protein 78 (grp78) coding sequence to about 175 base pairs 3′ of the site of initiation of transcription of the rat grp78 coding sequence;
- and
- b) a heterologous nucleic acid sequence operatively linked to the regulatory sequence, wherein the heterologous sequence comprises a structural gene that encodes a biologically active enzyme that converts a non-therapeutically effective compound to a therapeutically-effective compound in vivo.
Dependencies are inferred from the claim text.