Hafnia phytase

#Sequence Listing

#The present application contains a computer readable form of a sequence listing. The contents of the computer readable form are fully incorporated herein by reference.

#Deposit of Biological Material

#FIG. 1 shows the residual inositol-phosphate bound phosphorous after in vitro incubation in a comparison between the Hafnia alvei phytase and a Citrobacter braakii phytase dosed from 125 to 500 FYT/kg Feed.

#FIG. 2 shows a comparison of the residual inositol-phosphate bound phosphorous after in vitro incubation between the Hafnia alvei phytase and a Peniophora lycii phytase dosed at 250 FYT/kg Feed and 500 FYT/kg Feed.

#FIG. 3 shows the Appendix of the structural coordinates for the solved crystal three dimensional structure of the Hafnia alvei phytase.

#Phytase activity: In the present context a polypeptide having phytase activity (a phytase) is an enzyme which catalyzes the hydrolysis of phytate (myo-inositol hexakis-phosphate) to (1) myo-inositol and/or (2) mono-, di-, tri-, tetra- and/or penta-phosphates thereof and (3) inorganic phosphate.

#The ENZYME site at the internet (www.expasy.ch/enzyme/) is a repository of information relative to the nomenclature of enzymes. It is primarily based on the recommendations of the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology (IUB-MB) and it describes each type of characterized enzyme for which an EC (Enzyme Commission) number has been provided (Bairoch A. The ENZYME database, 2000, Nucleic Acids Res 28:304-305). See also the handbook Enzyme Nomenclature from NC-IUBMB, 1992).

#According to the ENZYME site, three different types of phytases are known: A 3-phytase (myo-inositol hexaphosphate 3-phosphohydrolase, EC 3.1.3.8), a 6-phytase (myo-inositol hexaphosphate 6-phosphohydrolase, EC 3.1.3.26), and a 5-phytase (EC 3.1.3.72). For the purposes of the present invention, all types are included in the definition of phytase.