Synthesis of 1,2,4-butanetriol enantiomers from carbohydrates
AssigneeBoard of Trustees of Michigan State University
InventorJohn W Frost
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Abstract
A bioengineered synthesis scheme for the production of L-1,2,4-butanetriol, D-1,2,4-butanetriol and racemic mixtures thereof from a carbon source is provided. Methods of producing L-1,2,4-butanetriol, D-1,2,4-butanetriol and racemic mixtures thereof are also provided. Methods are also provided for converting D-1,2,4-butanetriol and L-1,2,4,-butanetriol to D,L-1,2,4-butanetriol trinitrate.
Claims39
A process for making 1,2,4-butanetriol, comprising the steps of
- (a) converting D-xylose to D-xylonic acid by contacting the D-xylose with a D-xylose dehydrogenase enzyme derived from Pseudomonas;
- (b) converting the D-xylonic acid from step (a) to 3-deoxy-glycero-pentulosonic acid by contacting the D-xylonic acid with a D-xylonate dehydratase enzyme derived from Escherichia;
- (c) converting 3-deoxy-glycero-pentulosonic acid from step (b) to 3,4-dihydroxybutanal by contacting the 3-deoxy-glycero-pentulosonic acid with a benzylformate decarboxylase enzyme derived from Pseudomonas, Erwina, Acetobacter, Zymobacter, or Sacchararomyces;
- and
- (d) converting 3,4-dihydroxybutanal from step (c) to 1,2,4-butanetriol by contacting the 3,4-dihydroxybutanal with a dehydrogenase enzyme derived from an Escherichia.
A process for making 3,4-dihydroxybutanal, comprising culturing a microbe expressing 2-ketoacid decarboxylase enzyme derived from Pseudomonas, Erwina, Acetobacter, Zymobacter, or Sacchararomyces in the presence of 3-deoxy-glycero-pentulosonic acid, thereby converting 3-deoxy-glycero-pentulosonic acid to 3,4-dihydroxybutanal by contacting the 3-deoxy-glycero-pentulosonic acid with the 2-ketoacid decarboxylase.
Dependencies are inferred from the claim text.