Method of recovering lipase activity
AssigneeThe Nisshin OilliO Group, Ltd.
InventorsSatoshi Negishi, Junko Suzuki, Yoshie Yamauchi
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Abstract
The present invention discloses a method of recovering lipase activity which comprises the steps of using a lipase derived from Thermomyces sp. and immobilized on a carrier, or a lipase powder composition which comprises a filter aid and the lipase derived from Thermomyces sp. and immobilized on a carrier which is crushed into the average particle size of 1 μm or larger and smaller than 300 μm in an esterification or transesterification reaction; and washing said lipase or lipase powder composition with triacylglycerol. According to this method, the decreased lipase activity can be effectively recovered.
Claims9
A method of recovering lipase activity which comprises the steps of using a lipase derived from Thermomyces sp. and immobilized on a carrier, or a lipase powder composition which comprises a filter aid and the lipase derived from Thermomyces sp. and immobilized on a carrier which is crushed into an average particle size of 1 μm or larger and smaller than 300 μm in an esterification or transesterification reaction;
- and
- washing said lipase or lipase powder composition with triacylglycerol,
- wherein the triacylglycerol comprises at least one medium-chain fatty acid triglyceride.
A method of an esterification or transesterification reaction which comprises the steps of using a lipase derived from Thermomyces sp. and immobilized on a carrier, or a lipase powder composition which comprises a filter aid and the lipase derived from Thermomyces sp. and immobilized on a carrier which is crushed into an average particle size of 1 μm or larger and smaller than 300 μm in an esterification or transesterification reaction;
- separating the lipase or lipase powder composition from the reaction system, and washing the lipase or lipase powder with triacylglycerol to recover the lipase activity thereof;
- and then, conducting an esterification or transesterification reaction using the resulting immobilized lipase or lipase powder composition.
Dependencies are inferred from the claim text.