Δ-9 desaturase and its use in making polyunsaturated fatty acids
AssigneeE. I. Du Pont de Nemours and Company
InventorsJohn E. Seip, Quinn Qun Zhu
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Abstract
The present invention relates to a Δ9 desaturase, which has the ability to convert palmitic acid [16:0] or stearic acid [18:0] into palmitoleic acid [16:1] or oleic acid [18:1], respectively. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ9 desaturase along with a method of making long chain polyunsaturated fatty acids (PUFAs) using this Δ9 desaturase in oleaginous yeast is disclosed.
Claims19
An isolated nucleic acid molecule comprising:
- a.) an isolated nucleotide sequence encoding a Δ9 desaturase enzyme as set forth in SEQ ID NO:2;
- b.) an isolated nucleotide sequence encoding a Δ9 desaturase enzyme that hybridizes with (a) under the following hybridization conditions:
- 0.1×SSC, 0.1% SDS, 65° C. and washed with 2×SSC, 0.1% SDS followed by 0.1×SSC, 0.1% SDS;
- or
- c.) an isolated nucleotide sequence that is completely complementary to (a) or (b).
An isolated nucleic acid molecule comprising a first nucleotide sequence encoding a Δ9 desaturase enzyme of at least 340 amino acids that has at least 90% identity based on BLASTP algorithms when compared to a polypeptide having the sequence as set forth in SEQ ID NO:2;
- or a second nucleotide sequence comprising the complement of the first nucleotide sequence.
Dependencies are inferred from the claim text.