Method of use of a platelet/nucleated cell concentrate from bone marrow or blood to treat a bone defect
AssigneesPall Corporation, Smith & Nephew, Inc.
InventorsSamuel O. Sowemimo-Coker, Marcus Lee Scott, Marc Long, Ed Margerrison, Michael B. Cooper
Life of this patent
Estimating the term…
Abstract
The present invention is directed to methods and compositions regarding the preparation of an cell concentrate, such as, for example, an osteogenic cell concentrate, from a physiological solution, such as bone marrow aspirate, blood, or a mixture thereof. In specific embodiments, the invention provides methods and compositions utilizing two physiological solution-processing techniques, particularly in a point of care environment, wherein centrifugation is not employed.
Claims18
A method of treating a bone defect in an individual comprising the steps of:
- obtaining a physiological fluid comprising nucleated cells, platelets, plasma and red blood cells, which has not been previously subjected to centrifugation,
- preparing a cell concentrate from the physiological fluid comprising
- combining a hypotonic fluid with the physiological fluid, the hypotonic fluid being in an amount which does not cause the cells to lyse in the physiological fluid/hypotonic fluid combination,
- subjecting the combined physiological fluid/hypotonic fluid to a first filtration device to produce a filter retentate, which is a first product, and a permeate fluid, wherein said filter retentate comprises platelets and nucleated cells per unit volume greater than in the physiological fluid, and wherein said permeate fluid comprises plasma and red blood cells,
- removing the filter retentate from the filter, and
- subjecting the filter retentate to a second filtration device to produce a cell concentrate, which is a second product,
- delivering the second product to the bone defect in the individual.
Dependencies are inferred from the claim text.