Brain tissue damage therapies
AssigneeAcademia Sinica
InventorsHung Li, Woei-Cherng Shyu, Dah-Ching Ding, Shin-Zong Lin
Life of this patent
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Abstract
A cultured pluripotent animal cell that is CD13+, CD90+, and CD117−. Also disclosed are methods for making the cell and methods of treating a brain tissue damage and increasing the expression level of a neuraltrophic factor in a subject.
Claims13
A method of treating a brain tissue injury resulting from cerebral ischemia, comprising administering to a subject in need thereof a composition containing a plurality of cultured pluripotent cells that are CD13+, CD90+, and CD117−, resulting in an improved neurological behavior or brain metabolic activity in the subject as compared to before the treatment, wherein the cultured pluripotent cells are produced by the following procedure:
- obtaining Wharton's jelly from an umbilical cord of a human;
- culturing the Wharton's jelly in DMEM containing 10% human cord blood serum to allow cells to migrate out of the Wharton's jelly;
- and
- culturing the cells that migrated out of the Wharton's jelly to obtain the cultured pluripotent cells.
A method of treating neurodegeneration resulting from cerebral ischemia, the method comprising:
- identifying a subject suffering from or being at risk for developing neurodegeneration resulting from cerebral ischemia, and
- administering to the subject a composition containing a plurality of cultured pluripotent animal cells that are CD13+, CD90+, and CD 117−, resulting in an improved neurological behavior or brain metabolic activity in the subject as compared to before the treatment;
- wherein the cultured pluripotent cells are prepared by the following procedure:
- obtaining Wharton's jelly from an umbilical cord of a human;
- culturing the Wharton's jelly in DMEM containing 10% human cord blood serum to allow cells to migrate out of the Wharton's jelly;
- and
- culturing the cells that migrated out of the Wharton's jelly to obtain the cultured pluripotent cells.
A method of increasing the expression level of a neurotrophic factor in the brain of a subject suffering from cerebral ischemia, comprising administering to a subject in need thereof a composition containing a plurality of cultured pluripotent animal cells that are CD13+, CD90+, and CD117−resulting in an increased level of expression of a neurotrophic factor in the subject as compared to non-ischemic and vehicle controls, wherein the cultured pluripotent cells are prepared by the following procedure:
- obtaining Wharton's jelly from an umbilical cord of a human;
- culturing the Wharton's jelly in DMEM containing 10% human cord blood serum to allow cells to migrate out of the Wharton's jelly;
- and
- culturing the cells that migrated out of the Wharton's jelly to obtain the cultured pluripotent cells;
- wherein the expression level of the neurotrophic factor is measured in a biological sample obtained from the subject.
Dependencies are inferred from the claim text.