Process for producing a bio-pesticide composition containing Trichoderma harzianum and Pseudomonas fluorescens
AssigneesDepartment of Biotechnology, Indian Institute of Horticultural Research
InventorsMahendrakar Sreenivasa Rao, Nanjundagowda Ramachandran
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Abstract
A process for the production of organic and combination formulation of bio-pesticide containing Trichoderma harzianum and Pseudomonas fluorescens comprising preparation of mother culture, liquid fermentation as well as solid fermentation of T. harzianum, preparation of mother culture, liquid fermentation as well as solid fermentation of P. fluorescens separately, followed by mixing both the bio-pesticides in the proportion of 1-2: 1-2: preferably 1:1 to get the final combination, formulation.
Claims13
A process for the production of organic and combination formulation of bio-pesticide containing Trichoderma harzianum and Pseudomonas fluorescens comprising:
- preparing a first mother culture, comprising a liquid fermentation of T. harzianum and a solid fermentation of T harzianum, wherein the solid fermentation of T. harzianum comprises neem cake, pongamia cake and wheat bran;
- preparing a second mother culture comprising liquid fermentation and solid fermentation of P. fluorescens separately, wherein the solid fermentation of P. fluorescens comprises neem cake, pongamia cake and wheat bran;
- and
- mixing said first mother culture and said second mother culture in the proportion of 1-2:1-2 to produce a final combination formulation.
Dependencies are inferred from the claim text.