Porous ferro- or ferrimagnetic glass particles for isolating molecules
AssigneeQiagen GmbH
InventorsPhilippe Sauer, Bernd Springer, Thomas Manz, Christoph Ritt, Roland Fabis
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Abstract
Porous, ferro- or ferrimagnetic, glass particles are described that selectively bind molecules of interest, especially nucleic acid molecules, under appropriate conditions. Methods of preparing the porous, ferro- or ferrimagnetic, glass particles and their use for identifying or separating molecules of interest are also described. Kits comprising the porous, ferro- or ferrimagnetic, glass particles are also provided.
Claims17
A method of making porous, ferro- or ferrimagnetic, glass particles comprising:
- a) providing a suspension of ferro- or ferrimagnetic iron oxide particles,
- b) adding a tetraalkoxysilane to the suspension of the ferro- or ferrimagnetic iron oxide particles at a pH between 6 and 8,
- c) hydrolyzing the tetraalkoxysilane and precipitating silica glass on the ferro- or ferrimagnetic iron oxide particles by adding a buffer having a pH lower than 6 or higher than 9 to the suspension to form ferro- or ferrimagnetic, porous glass particles,
- d) separating the resulting porous, ferro- or ferrimagnetic, glass particles from suspension,
- e) washing the separated porous, ferro- or ferrimagnetic, glass particles with an anhydrous alcohol, and
- f) drying the porous, ferro- or ferrimagnetic, glass particles at a temperature below the Curie temperature, wherein the drying is at 200° C. to about 500° C.
A method of making porous, ferro- or ferrimagnetic, glass particles comprising:
- a) providing a suspension of ferro- or ferrimagnetic iron oxide particles in a glycerol solution that has a pH of 5 or less or a pH of 9 or greater,
- b) adding a tetraethoxysilane dropwise with stirring to the suspension of the ferro- or ferrimagnetic iron oxide particles within 10 minutes,
- c) aging the mixture to allow the ferro- or ferrimagnetic porous glass particles to solidify and stabilize,
- d) separating the resulting porous, ferro- or ferrimagnetic, glass particles from the liquid by applying an external magnetic field,
- e) washing the separated porous, ferro- or ferrimagnetic, glass particles with an anhydrous alcohol, and
- f) drying the porous, ferro- or ferrimagnetic, glass particles at a temperature between about 3000 and 500° C.
A method of making porous, ferro- or ferrimagnetic, glass particles comprising:
- a) providing a suspension of ferro- or ferrimagnetic iron oxide particles in alcohol,
- b) adding silica nanoparticles to the suspension of the ferro- or ferrimagnetic iron oxide particles at a pH lower than 6,
- c) aging the mixture by continuous stirring,
- d) separating the resulting porous, ferro- or ferrimagnetic, glass particles from the liquid,
- e) washing the separated porous, ferro- or ferrimagnetic, glass particles, and
- f) drying the porous, ferro- or ferrimagnetic, glass particles at a temperature at about 200° C.
A method of making porous, ferro- or ferrimagnetic, glass particles comprising:
- a) providing a suspension of ferro- or ferrimagnetic iron oxide particles in paraffin oil
- b) adding an aqueous sodium silicate solution, to the suspension of the ferro- or ferrimagnetic iron oxide particles at a pH lower than 6,
- c) aging the mixture by continuous stirring,
- d) separating the resulting porous, ferro- or ferrimagnetic, glass particles from the liquid,
- e) washing the separated porous, ferro- or ferrimagnetic, glass particles, and
- f) drying the porous, ferro- or ferrimagnetic, glass particles at a temperature at about 200° C.
Dependencies are inferred from the claim text.