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US 7,922,891 B2Granted
Methods for detection and quantification of analytes
AssigneeArizona Board of Regents
InventorsJohn Devens Gust, Jr., Ana L. Moore, Thomas A. Moore, Alicia Brune
Life of this patent
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Abstract
The present invention relates to a method for detecting and quantitating NADH/NAD+ and/or NADPH/NADP+ as well as NADHJNAD+ and/or NADPH/NADP+ dependent enzymes using a photoelectrochemical cell.
Claims18
A method for detecting and quantifying the amount of NADH in a mixture, comprising the steps of:
- providing an electrochemical fuel cell comprising:
- a. a first electrochemical half-cell comprising a dye-sensitized nanoparticulate photoanode operating in an aqueous medium and NADH;
- b. a second electrochemical half cell comprising a suitable cathode;
- c. a device for detection of electrical current passing between the photoanode and the cathode and the associated voltage;
- and
- d. a suitable light source;
- illuminating the photoanode with light;
- consuming the NADH by the electrochemical fuel cell;
- detecting a photocurrent flowing through the device;
- and
- quantifying the amount of NADH.
8 more elements
A method for detecting and quantifying the amount of NADPH in a mixture, comprising the steps of:
- providing an electrochemical fuel cell comprising:
- a. a first electrochemical half-cell comprising a dye-sensitized nanoparticulate photoanode operating in an aqueous medium and NADPH;
- b. a second electrochemical half cell comprising a suitable cathode;
- c. a device for detection of electrical current passing between the photoanode and the cathode and the associated voltage;
- and
- d. a suitable light source;
- illuminating the photoanode with light;
- consuming the NADPH by the electrochemical fuel cell;
- detecting a photocurrent flowing through the device;
- and
- quantifying the amount of NADPH.
8 more elements
A method for detecting and quantifying the amount of an analyte used as a substrate by an NAD+-dependent enzyme, comprising the steps of:
- providing an electrochemical fuel cell comprising:
- a. a first electrochemical half-cell comprising a dye-sensitized nanoparticulate photoanode operating in an aqueous medium, which contains NAD+ and an enzyme that uses the analyte of interest as a substrate;
- b. a second electrochemical half cell comprising a cathode;
- c. an ammeter or other suitable device for detection of electrical current passing between the photoanode and the cathode and the associated voltage;
- and
- d. a suitable light source;
- illuminating the photoanode with light;
- detecting a first photocurrent flowing through the device;
- adding the analyte to the photoanode;
- illuminating the photoanode with light;
- consuming the NAD+ by the electrochemical fuel cell;
- detecting a second photocurrent flowing through the device;
- and
- quantifying the amount of analyte.
11 more elements
A method for detecting and quantifying the amount of an analyte used as a substrate by an NADP+-dependent enzyme, comprising the steps of:
- providing an electrochemical fuel cell comprising:
- a. a first electrochemical half-cell comprising a dye-sensitized nanoparticulate photoanode operating in an aqueous medium, which contains NADP+ and an enzyme that uses the analyte of interest as a substrate;
- b. a second electrochemical half cell comprising a cathode;
- c. an ammeter or other suitable device for detection of electrical current passing between the photoanode and the cathode and the associated voltage;
- and
- d. a suitable light source;
- illuminating the photoanode with light;
- detecting a first photocurrent flowing through the device;
- adding the analyte to the photo anode;
- illuminating the photoanode with light;
- consuming the NADP+ by the electrochemical fuel cell;
- detecting a second photocurrent flowing through the device;
- and
- quantifying the amount of analyte.
11 more elements
A method for detecting and quantifying the amount of an analyte used as a substrate by an NADH-dependent enzyme, comprising the steps of:
- providing an electrochemical fuel cell comprising:
- a. a first electrochemical half-cell comprising a dye-sensitized nanoparticulate photoanode operating in an aqueous medium, which contains NADH and an enzyme that uses the analyte of interest as a substrate;
- b. a second electrochemical half cell comprising a suitable cathode;
- c. an ammeter or other suitable device for detection of electrical current passing between the photoanode and the cathode and the associated voltage;
- and
- d. a suitable light source;
- illuminating the photoanode with light;
- detecting a first photocurrent flowing through the device;
- adding the analyte to the photoanode;
- illuminating the photoanode with light;
- consuming the NADH by the electrochemical fuel cell;
- detecting a second photocurrent flowing through the device;
- and
- quantifying the amount of analyte.
11 more elements
A method for detecting and quantifying the amount of an analyte used as a substrate by an NADPH-dependent enzyme, comprising the steps of:
- providing an electrochemical fuel cell comprising:
- a. a first electrochemical half-cell comprising a dye-sensitized nanoparticulate photoanode operating in an aqueous medium, which contains NADPH and an enzyme that uses the analyte of interest as a substrate;
- b. a second electrochemical half cell comprising a suitable cathode;
- c. an ammeter or other suitable device for detection of electrical current passing between the photoanode and the cathode and the associated voltage;
- and
- d. a suitable light source;
- illuminating the photoanode with light;
- detecting a first photocurrent flowing through the device;
- adding the analyte to the photoanode;
- illuminating the photoanode with light;
- consuming the NADPH by the electrochemical fuel cell;
- detecting a second photocurrent flowing through the device;
- and
- quantifying the amount of analyte.
11 more elements
Dependencies are inferred from the claim text.