Enhanced delivery of naked DNA to skin by non-invasive in vivo electroporation
AssigneeGenetronics, Inc.
InventorsLei Zhang, Dietmar P. Rabussay
Life of this patent
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Abstract
In vivo methods are provided for using an electric field to delivery therapeutic or immunizing treatment to a subject by applying non-invasive, user-friendly electrodes to the surface of the skin. Thus, therapeutic or immunizing agents can be delivered into cells of skin for local and systemic treatments or for immunization with optimal gene expression and minimal tissue damage. In particular, therapeutic agents include naked or formulated nucleic acid, polypeptides and chemotherapeutic agents.
Claims20
A method for delivering an isolated polynucleotide species that encodes a therapeutic gene product into skin cells of a subject, comprising:
- a) introducing about 0.05 μg to about 100 μg of at least one isolated polynucleotide species encoding a therapeutic gene product into each of one or more skin tissue sites of a subject;
- and
- b) substantially contemporaneously with the introduction of the isolated polynucleotide species, applying one or more electric pulses across at least two noninvasive electrodes to generate a total charge transfer of about one mC to about 1000 mC per skin tissue site so as to allow the polynucleotide species to enter cells within the skin tissue site, thereby delivering the isolated polynucleotide species into skin cells of the subject.
Dependencies are inferred from the claim text.