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US 7,919,277 B2Granted
Detection and typing of bacterial strains
AssigneeDanisco A/S
InventorsW. Michael Russell, Rodolphe Barrangou, Philippe Horvath
Life of this patent
Estimating the term…
Abstract
Methods for the detection and typing of bacterial strains from food products and dietary supplements, environmental samples, in vivo/in vitro samples, and for studying the natural diversity of the species are disclosed. Potential applications also include product development and/or detection and differentiation of new bacterial strains.
Claims8
A method for detecting the presence of a Lactobacillus acidophilus in a sample, comprising:
- a) obtaining a sample;
- b) amplifying a region of DNA comprising repetitive sequences that are at least 86% identical to the repetitive sequence comprised in the intergenic region set forth in SEQ ID NO:1 in said sample, to create amplified DNA;
- and,
- c) detecting said amplified DNA.
1 more element
A method for typing a Lactobacillus acidophilus bacterial strain comprising:
- a) obtaining a sample;
- b) amplifying a region of DNA comprising repetitive sequences that are at least 86% identical to the repetitive sequences comprised in the intergenic region set forth in SEQ ID NO:1 in said sample to create amplified DNA;
- and
- c) typing said bacterial strain based on said amplified DNA.
1 more element
A method for typing a Lactobacillus acidophilus bacterial strain comprising:
- a) obtaining a sample comprising said Lactobacillus acidophilus bacterial strain;
- b) amplifying a region of DNA comprising repetitive sequences that are at least 86% identical to the repetitive sequences comprised in the intergenic region set forth in SEQ ID NO:1 in said sample to create amplified DNA;
- c) adding to said amplified DNA at least one restriction enzyme that recognizes one or more sites in said amplified DNA;
- d) incubating said restriction enzyme with said amplified DNA for a time sufficient to form restriction fragments;
- e) determining the number of said restriction fragments and their size;
- and
- f) typing said bacterial strain based on said number and size of said restriction fragments.
4 more elements
Dependencies are inferred from the claim text.