Electrocapturing flow cell
AssigneeGeorge Mason Intellectual Properties, Inc.
InventorVictor Morozov
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Abstract
A flow cell for electrophoretically-assisted capturing analytes from a flow. The flow cell includes a specimen chamber, a first membrane, a second membrane, a first electrode chamber, and a second electrode chamber. The specimen chamber may have a sample inlet and a sample outlet. A first portion of the first membrane may be coupled to a first portion of the specimen chamber. A first portion of the second membrane may be coupled to a second portion of the specimen chamber. The first electrode chamber may be configured to accept a charge. A portion of the first electrode chamber may be coupled to a second portion of the first membrane. A second electrode chamber may be configured to accept an opposite charge. A portion of the second electrode chamber may be coupled to a second portion of the second membrane.
Claims21
A flow cell comprising:
- a specimen chamber, the specimen chamber having:
- a sample inlet;
- and
- a sample outlet;
- a first membrane, a first portion of the first membrane coupled to a first portion of the specimen chamber;
- a second membrane, a first portion of the second membrane coupled to a second portion of the specimen chamber;
- a first chamber configured to accept a charge, a portion of the first chamber coupled to a second portion of the first membrane;
- and
- a second chamber configured to accept an opposite charge, a portion of the second chamber coupled to a second portion of the second membrane,
- wherein at least a part of the first portion of at least one of the first membrane and the second membrane is configured to bind an analyte from fluid flow in the specimen chamber under electric force, and is visible from outside the specimen chamber through a transparent portion.
A method for electro-capturing analytes from a flow comprising:
- pumping an analyte solution:
- into a specimen chamber through a sample inlet;
- over a microarray on at least a part of a second portion of at least one of a first membrane and a second membrane configured to bind an analyte from fluid flow in the specimen chamber under electric force;
- and
- out of the specimen chamber through a sample outlet;
- applying an electric charge to a first electrode, the first electrode disposed in a first electrode chamber;
- a portion of the first electrode chamber coupled to a first portion of a first membrane, the second portion of the first membrane coupled to a first portion of the specimen chamber;
- applying an opposite electric charge to a second electrode, the second electrode disposed in a second electrode chamber;
- a portion of the second electrode chamber coupled to a first portion of a second membrane, the second portion of the second membrane coupled to a second portion of the specimen chamber,
- wherein at least the part of the second portion of at least one of the first membrane and the second membrane is visible from outside the specimen chamber through a transparent portion.
Dependencies are inferred from the claim text.