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US 7,915,579 B2Granted
Method and apparatus of liquid sample-desorption electrospray ionization-mass specrometry (LS-DESI-MS)
AssigneeOhio University
InventorsHao Chen, Zhixin Miao
Life of this patent
Estimating the term…
Abstract
An apparatus and method for direct analysis of continuous-flow liquid samples by desorption electrospray ionization-mass spectrometry (DESI-MS) including a sample stage that is adapted to receive a liquid sample and a nebulizing ionizer that is configured to generate a charged, nebulized solvent and thereby desorb at least a portion of the liquid sample from the sample stage.
Claims24
A liquid sample ionizer comprising:
- a fluid conduit configured to continuously supply a liquid sample;
- a sample stage configured to receive the liquid sample from the fluid conduit;
- and
- a nebulizing ionizer configured to generate a charged, nebulized solvent and to direct the charged, nebulized solvent onto the liquid sample on the sample stage, wherein the charged, nebulized solvent desorbs at least a portion of the liquid sample from the sample stage.
1 more element
A mass spectrometer comprising:
- a fluid conduit configured to continuously supply a liquid sample for ionization and analysis by the mass spectrometer;
- an ion source comprising a sample stage configured to receive the liquid sample and a nebulizing ionizer configured to generate a charged, nebulized solvent, wherein the charged, nebulized solvent desorbs at least a portion of the liquid sample from the sample stage;
- a mass analyzer configured to receive the desorbed portion of the liquid sample, to ionize the desorbed portion of the liquid sample, and to analyze a mass-to-charge ratio of the ionized, desorbed portion of the liquid sample;
- and
- a controller configured to operate the ion source, the mass analyzer, or a combination thereof.
2 more elements
A method of ionizing a liquid sample for mass spectroscopy analysis comprising:
- generating a charged, nebulized solvent;
- continuously supplying a liquid sample;
- directing the charged, nebulized solvent to the liquid sample thereby desorbing at least a portion of the liquid sample;
- ionizing the desorbed portion of the liquid sample;
- and
- directing the ionized, desorbed portion of the liquid sample to a mass analyzer.
3 more elements
A method of analyzing a liquid sample comprising:
- continuously introducing a liquid sample to a sample stage;
- generating a charged, nebulized solvent;
- directing the charged, nebulized solvent to the liquid sample on the sample stage, wherein the charged, nebulized solvent desorbs at least a portion of the liquid sample from the sample stage and directs the desorbed portion of the liquid sample in a direction substantially toward a mass analyzer;
- ionizing the desorbed portion of the liquid sample;
- separating an ionized solvent from the ionized, desorbed portion of the ionized liquid sample;
- and
- analyzing a mass-to-charge ratio of the desorbed portion of the ionized sample.
4 more elements
Dependencies are inferred from the claim text.