Culture systems for ex vivo development
AssigneeAdvanced Cell Technology, Inc.
InventorsMichael D. West, Karen B. Chapman, Irina V. Klimanskaya
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Abstract
The present invention provides methods for the culture of animal pluripotent stem cells and their differentiated progeny cells, tissues, and organs, and nonhuman animal embryos and fetuses.
Claims14
A method of maintaining human pluripotent stem cells in an undifferentiated state comprising co-culturing said human pluripotent stem cells with chick embryonic fibroblasts feeder cells, wherein the chick embryonic fibroblast feeder cells are obtained from an eviscerated embryo with the embryo's head left on.
A method of maintaining human pluripotent stem cells in an undifferentiated state comprising co-culturing said human pluripotent stem cells with chick embryonic fibroblasts feeder cells, wherein the chick embryonic fibroblast feeder cells are used at passage two.
A method of maintaining human pluripotent stem cells in an undifferentiated state comprising co-culturing said human pluripotent stem cells with chick embryonic fibroblasts feeder cells, wherein the chick embryonic fibroblast feeder cells were derived from a 7 or 8-day embryo.
Dependencies are inferred from the claim text.