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US 7,910,304 B2Granted
Random array DNA analysis by hybridization
AssigneeCallida Genomics, Inc.
InventorRadoje T. Drmanac
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Abstract
The invention relates to methods and devices for analyzing single molecules, i.e. nucleic acids. Such single molecules may be derived from natural samples, such as cells, tissues, soil, air and water without separating or enriching individual components. In certain aspects of the invention, the methods and devices are useful in performing nucleic acid sequence analysis by probe hybridization.
Claims14
A method for sequencing target nucleic acids, said method comprising:
- (a) providing a random array of said target nucleic acids;
- (b) hybridizing a first and second population of oligonucleotide probes to said array;
- (c) ligating oligonucleotide probes from said first and second populations that are hybridized to adjacent positions on said target nucleic acids to produce ligated probes;
- (d) collecting a signal produced by said ligated probes;
- (e) analyzing said signal to identify at least one nucleotide of said target nucleic acids;
- (f) repeating steps (b)-(e) to provide multiple cycles of sequence information for said target nucleic acids, such that at least one nucleotide of said target nucleic acids is identified in more than one cycle;
- (g) assembling said multiple cycles of sequence information to provide sequences of all or a portion of said target nucleic acids.
4 more elements
Dependencies are inferred from the claim text.