Increased bacterial CoA and acetyl-CoA pools
AssigneeRice University
InventorsKa-Yiu San, George Nelson Bennett, Ravishankar V. Vadali
Life of this patent
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Abstract
Methods of increasing the cellular pool of A-CoA and thus driving the metabolic pathways in the direction of A-CoA containing metabolites by overexpressing rate limiting enzymes in A-CoA synthesis. Methods of increasing intracellular levels of CoA and A-CoA through genetic engineering of bacterial strains in conjunction with supplementation with precursor molecules.
Claims9
A bacterium comprising a recombinant bacterial panK gene overexpressing pantothenate kinase (PANK), a recombinant bacterial pdh gene overexpressing pyruvate dehydrogenase (PDH), and a recombinant yeast atf gene overexpressing alcohol acetyl transferase (ATF),
- a) wherein said bacteria has more PANK, PDH and ATF activity than a bacteria without said recombinant panK, pdh, or atf genes,
- b) wherein said bacterium produces increased flux through the Acetyl-CoA node when grown in culture supplemented with pantothenate.
An E. coli comprising:
- a) one or more expression vectors that overexpresses wild type E. coli pantothenate kinase (PANK), E. coli pyruvate dehydrogenase (PDH), and yeast alcohol acetyl transferase (ATF), as compared with a bacteria that lacks said one or more expression vectors;
- and
- b) deletions in both the ackA gene and the pta gene;
- wherein said E. coli produces increased flux through the Acetyl-CoA node when grown in culture supplemented with pantothenate, as compared with said E. coli without said expression vectors or said deletions.
Dependencies are inferred from the claim text.