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US 7,901,708 B2Granted
Liposomal apparatus and manufacturing methods
AssigneeProtiva Biotherapeutics, Inc.
InventorsIan MacLachlan, Lloyd Brian Jeffs, Lorne R. Palmer, Cory Giesbrecht
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Abstract
The present invention provides apparatus and processes for producing liposomes. By providing a buffer solution in a first reservoir, and a lipid solution in a second reservoir, continuously diluting the lipid solution with the buffer solution in a mixing chamber produces a liposome. The lipid solution preferably comprises an organic solvent, such as a lower alkanol.
Claims22
A process for producing a lipid vesicle encapsulating a nucleic acid within the lipid vesicle, said process comprising:
- providing an aqueous solution including a nucleic acid in a first reservoir;
- providing an organic lipid solution in a second reservoir, wherein the lipids present in said organic lipid solution are solubilized in a lower alkanol at a concentration of about 75% v/v to 100% v/v;
- and
- mixing said organic lipid solution with said aqueous solution by introducing said organic lipid solution and said aqueous solution into a mixing environment at about equal flow rates;
- wherein said mixing instantaneously produces a lipid vesicle encapsulating the nucleic acid within the lipid vesicle by diluting said lower alkanol to a concentration of between 45% v/v to about 60% v/v;
- and
- wherein the mixing environment includes a T-connector, wherein the aqueous solution and the organic lipid solution are introduced into the T-connector as opposing flows at about 180° relative to each other and mixed within the T-connector.
4 more elements
Dependencies are inferred from the claim text.