Barley Lipoxygenase 1 Gene, method of selecting barley variety, material of malt alcoholic drinks and process for producing malt alcoholic drink
AssigneeSapporo Breweries Limited
InventorsNaohiko Hirota, Takafumi Kaneko, Hisao Kuroda, Hirotaka Kaneda, Kiyoshi Takoi, Kazuyoshi Takeda
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Abstract
A selection method for barley lipoxygenase-1 deficient barley, comprising a step of distinguishing the barley lipoxygenase-1 deficient barley by whether or not the guanine at the splicing donor site of the 5th intron of the barley lipoxygenase-1 gene is mutated to a different base; and a method for production of malt alcoholic beverages using a material for malt alcoholic beverages derived from barley obtained by the selection method.
Claims17
An isolated barley lipoxygenase-1 mutant gene, wherein the guanine at the splicing donor site (5′-GT-3′) of the 5th intron of the barley lipoxygenase-1 gene is not guanine.
A selection method for barley lipoxygenase-1 deficient barley, the method comprising determining the presence or absence of a guanine at the splicing donor site of the 5th intron of the barley lipoxygenase-1 gene, and selecting the barley having an adenine, thymine or cytosine at the splicing donor site.
An isolated nucleic acid comprising the nucleotide sequence from position 1 to 1554 as set forth in SEQ ID NO:
- 10.
An isolated nucleic acid comprising the nucleotide sequence as set forth in SEQ ID NO:
- 11.
An isolated nucleic acid comprising the nucleotide sequence of 10 to 60 continuous bases including the 3178th base in the nucleotide sequence as set forth in SEQ ID NO:
- 11.
A method for detecting the presence of lipoxygenase-1 (LOX-1) activity in barley, the method comprising
- isolating genomic DNA from a barley sample, and
- detecting the presence or absence of the 3178th base of SEQ ID NO:
- 11, wherein the presence of the base is an indicator of the presence of LOX-1 activity in the barley.
Dependencies are inferred from the claim text.