Method and material for enhanced tissue-biomaterial integration

A hydrogel produced by a method comprising:

• (a) priming the surface of a bone by treating the bone with a priming agent comprising a polysaccharide with at least one produced aldehyde group and a first at least one free radical polymerizable group wherein the aldehyde is produced by treating said polysaccharide with periodate, to create a primed bone, wherein the least one produced aldehyde group reacts with the extracellular matrix of the bone to covalently bind the extracellular matrix of the bone and the priming agent;
• (b) adding to the primed bone a polymerizable agent, wherein the polymerizable agent comprises a second at least one free radical polymerizable group;
• and
• (c) reacting the primed bone and the polymerizable agent by a radical reaction to create a hydrogel covalently bound to the bone;
• wherein the radical reaction is between the first and second free radical polymerizable groups, and the first and second free radical polymerizable groups are each independently selected from the group consisting of an acrylate, diacrylate, oligoacrylate, methacrylate, dimethacrylate, and oligo methacrylate.

A method of integrating a hydrogel to the extracellular matrix of an osteochondral defect, comprising the steps of:

• (a) priming the surface of an osteochondral defect by treating the osteochondral defect with a priming agent to create a primed osteochondral defect, wherein the priming agent comprises a polysaccharide with at least one produced aldehyde group and a first at least one free radical polymerizable group wherein the aldehyde is produced by treating said polysaccharide with periodate, and the least one produced aldehyde group reacts with the extracellular matrix of the osteochondral defect to covalently bind the extracellular matrix of the osteochondral defect and the priming agent;
• (b) adding to the primed osteochondral defect a polymerizable agent, wherein the polymerizable agent comprises a second at least one free radical polymerizable group;
• and
• (c) reacting the primed osteochondral defect and the polymerizable agent by a radical reaction to create a hydrogel covalently bound to the osteochondral defect;
• wherein the radical reaction is between the first and second free radical polymerizable groups, and the first and second free radical polymerizable groups are each independently selected from the group consisting of an acrylate, diacrylate, oligoacrylate, methacrylate, dimethacrylate, and oligo methacrylate.

A hydrogel produced by a method comprising:

• (a) priming the surface of an osteochondral defect by treating the osteochondral defect with a priming agent comprising a polysaccharide with at least one produced aldehyde group and a first at least one free radical polymerizable group wherein the aldehyde is produced by treating said polysaccharide with periodate, to create a primed osteochondral defect, wherein the least one produced aldehyde group reacts with the extracellular matrix of the osteochondral defect to covalently bind the extracellular matrix of the osteochondral defect and the priming agent;
• (b) adding to the primed osteochondral defect a polymerizable agent, wherein the polymerizable agent comprises a second at least one free radical polymerizable group;
• and
• (c) reacting the primed osteochondral defect and the polymerizable agent by a radical reaction to create a hydrogel covalently bound to the osteochondral defect;
• wherein the radical reaction is between the first and second free radical polymerizable groups, and the first and second free radical polymerizable groups are each independently selected from the group consisting of an acrylate, diacrylate, oligoacrylate, methacrylate, dimethacrylate, and oligo methacrylate.