Culture medium for detecting and identifying Vibrio bacteria
AssigneeBiomerieux
InventorsDaniel Monget, Denis Robichon
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Abstract
The present invention relates to a reaction medium for cholerae-group Vibrio (cholerae/vulnificus and mimicus) and Vibrio parahaemolyticus bacteria, comprising a substrate for detecting a β-galactosidase enzymatic activity, a sugar, a coloured indicator. The invention also relates to the use of this medium for isolating and identifying cholerae-group Vibrio (cholerae/vulnificus and mimicus) and Vibrio parahaemolyticus. Finally, the invention relates to a method for identifying Vibrio cholerae and Vibrio parahaemolyticus bacteria, according to which beta-galactosidase activity is detected for identifying Vibrio cholerae and the acidification of a sugar is detected for revealing Vibrio parahaemolyticus.
Claims10
A culture and identification medium for distinguishing between cholerae-group Vibrio (cholerae/vulnificus and mimicus) and Vibrio parahaemolyticus bacteria, comprising:
- a substrate for detecting a β-galactosidase enzymatic activity,
- L-arabinose, and
- a neutral red or bromothymol blue colored indicator.
A method for isolating and identifying cholerae-group Vibrio and Vibrio parahaemolyticus, comprising:
- culturing microorganisms on a reaction medium, the reaction medium comprising:
- a substrate for detecting a β-galactosidase enzymatic activity,
- L-arabinose, and
- a neutral red or bromothymol blue colored indicator;
- and
- reading the reaction medium to detect cholerae-group Vibrio and Vibrio parahaemolyticus.
Dependencies are inferred from the claim text.