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US 7,892,772 B2Granted
Targeted ubiquitination of proteins and screening methods using a new class of ubiquitin ligase proteins
AssigneeITI Scotland Limited
InventorsJohn Roy Rohde, Claude Rene Raoul Parsot, Philippe Joseph Sansonetti
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Abstract
Members of the IpaH superfamily constitute a novel class of E3 ubiquitin ligases which are useful for engineering products which modulate trafficking and destruction of target proteins inside a cell and useful targets for identifying new antimicrobial molecules which modulate, especially inhibit, E3 ligases.
Claims4
A method for identifying a molecule which modulates the ligase activity of a member of the IpaH superfamily E3 ubiquitin ligase containing the sequence Cys-(Xaa)n-Asp (SEQ ID NO:
- 1), wherein “n” represents at least one up to 20 amino acids, comprising:
- contacting a test molecule with the E3 ubiquitin ligase in the presence of ubiquitin, E1 and E2 and a substrate protein;
- and
- determining ubiquitination of the substrate protein;
- wherein the E3 ubiquitin ligase is a Shigella E3 ubiquitin ligase.
2 more elements
Dependencies are inferred from the claim text.