System and method for high numeric aperture imaging systems

For imaging a target object, an imaging system comprising:

• (a) a first imaging sub-system comprising a light collection system positioned with respect to the target object in a first orientation, the first imaging sub-system configured to transmit a first image of the target object;
• (b) a first detector positioned to receive the first image of the target object at a first location on the first detector when the target object is in a first target location and to receive the first image of the target object at a second location on the first detector when the target object is in a second location different than the first location of the target object;
• (c) a second imaging sub-system comprising a light collection system positioned with respect to the target object in a second orientation different than the first orientation, the second imaging sub-system configured to transmit a second image of the target object at a best focus distance from the second imaging sub-system;
• (d) a second detector positioned to receive the second image of the target object;
• and
• (e) a processor communicatively linked to the first detector and configured to determine a distance between the first location on the first detector and the second location on the first detector, the processor communicatively linked to the second imaging sub-system and configured to transmit instructions to the second imaging sub-system based upon the determined distance between the first location on the first detector and the second location on the first detector, the second imaging sub-system configured to change the best focus distance from the second imaging sub-system based upon the instructions received from the processor to correct focus.