Spatially-resolved measurement method for the detection of melanin in fluorophor mixtures in a solid sample
AssigneeLTB-Lasertechnik Berlin GmbH
InventorsMatthias Scholz, Dieter Leupold
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Abstract
A method of obtaining a spatially resolved measurement for the detection of melanin in fluorophore mixtures of a solid sample includes emitting at least one pulse of light from a laser light source onto the solid sample at an excitation wavelength between 300 nm and 1000 nm so as to provide fluorescence excitation of the melanin by photon absorption. The pulse of light has a photon flux density between 1026 to 1028 photons per cm2 per second and a pulse length between 0.5 ns and 5 ns. An emitted spectral fluorescence response of the melanin is provided by evaluating a number of photons emitted at fluorescence wavelengths between 400 nm and 700 nm as to identify the melanin.
Claims20
A method of obtaining a spatially resolved measurement for detection of melanin in a fluorophore mixture of a solid sample, the method comprising:
- emitting at least one pulse of light from a laser light source onto the solid sample so as to provide fluorescence excitation of the melanin by photon absorption, wherein the at least one pulse of light has a photon flux density between 1026 to 1028 photons per cm2 per second, a pulse length between 0.5 ns and 5 and an excitation wavelength between 300 nm and 1000 nm;
- and
- providing an emitted spectral fluorescence response of the melanin by evaluating a number of photons emitted at fluorescence wavelengths between 400 nm and 700 nm so as to identify the melanin.
Dependencies are inferred from the claim text.