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AssigneeKorea Research Institute of Bioscience and Biotechnology
InventorsJang Ryol Liu, Hwa-Jee Chung, Sung Ran Min, Won Joong Jeong, Ju Young Park
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The present invention relates to a plastid transformation system capable of preventing second recombination events of heterologous genes inserted into plastid genomes. More specifically, this invention relates to a plastid transformation vector carrying a promoter and a terminator derived from organisms other than tobacco. The inventive recombinant expression vector for plastid transformation is capable of mass-producing exogenous proteins on a level par with conventional vectors carrying promoter/terminator couples of tobacco origin. At the same time, it is capable of preventing second recombination events within plastids. Thus, the vector of this invention is greatly useful in producing transgenic plants since it can effect a secure introduction of heterologous genes and support normal transformation and heterologous gene expression.
A recombinant expression vector for plastid transformation comprising an expression construct sequentially comprising a promoter, a ribosome binding site sequence and a terminator, wherein the promoter and terminator are exogenous to a target plant to be transformed, wherein the promoter has less than 50% homology to the plastid genome of the target plant, and wherein the terminator is a rrnB1/B2 terminator, wherein the expression construct is flanked by DNA sequences homologous to sequences in the plastid genome of the target plant.
Dependencies are inferred from the claim text.