Back to results
US 7,888,461 B2Granted
Snapin and methods for regulation of microtubule assembly and dendrite growth and branching
OwnerBonnie L. Firestein-Millerinventor
Life of this patent
Estimating the term…
Abstract
Fragments of snapin important for interaction with cypin and thus regulating microtubule assembly are provided. Also provided are methods of use of said fragments and kits to facilitate said methods.
Claims11
An isolated peptide consisting of the amino acids 50-136 of the amino acid sequence of SEQ ID NO:
- 1, wherein the isolated peptide binds to a peptide comprising an amino acid sequence of SEQ ID NO:
- 4.
An isolated peptide consisting of the amino acid sequence of SEQ ID NO:
- 3, wherein the isolated peptide binds to a peptide comprising an amino acid sequence of SEQ ID NO:
- 4.
An isolated peptide consisting of the amino acid sequence of SEQ ID NO:
- 2, wherein the isolated peptide binds to a peptide comprising an amino acid sequence of SEQ ID NO:
- 4.
A screening method for identifying a microtubule assembly inhibiting compound, the method comprising:
- obtaining at least one test sample and at least one control sample, wherein the at least one test sample and the at least one control sample comprise:
- 1) a snapin of SEQ ID NO:
- 1 or a fragment thereof comprising SEQ ID NO:
- 2, and
- 2) a cypin or a fragment thereof comprising SEQ ID NO:
- 4;
- adding a compound to be tested for the microtubule inhibiting activity to the at least one test sample;
- measuring in the at least one test sample and the at least one control sample an amount of binding of the snapin or the fragment thereof to the cypin or the fragment thereof,
- whereby a microtubule assembly inhibiting compound is identified by a measurement of an increase in the amount of binding of the snapin or the fragment thereof to the cypin or the fragment thereof in the at least one test sample as compared to the amount of binding in the at least one control sample.
6 more elements
Dependencies are inferred from the claim text.