Targeted gene deletions for polysaccharide slime formers
AssigneeCP Kelco U.S., Inc.
InventorsNancy E. Harding, Yamini N. Patel, Russell J. Coleman
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Abstract
The present invention provides improved diutan polysaccharides produced by mutant Sphingomonas strains containing at least one genetic modification that favors slime-forming diutan production that exhibits increased viscosity. Methods of making the mutant Sphingomonas strains and the culture broth containing such mutant Sphingomonas strains are also provided.
Claims14
A composition comprising a diutan polysaccharide which is produced by a process comprising recovering a diutan from a mutant Sphingomonas strain which contains at least one genetic modification that favors the production of diutan in a slime form, wherein said diutan as naturally produced by said mutant Sphingomonas strain imparts to a fluid an increased viscosity relative to an equivalent amount of diutan as naturally produced by a wild-type Sphingomonas strain ATCC 53159 wherein said gene mutations or deletions are in one or both of dpsN and dpsM genes and the Sphingomonas strain has deletion mutations in either or both dpsN and dpsM genes.
Dependencies are inferred from the claim text.