US 7,888,311 B2 · Uranium-chelating peptides and uses thereof

Abstract

The invention concerns uranium-chelating peptides as well as their uses for decontaminating soils and water, and for detecting and treating people contaminated by uranium. Said peptides have a helix-loop-helix type structure comprising the sequence of a calmodulin loop including at least one mutation of neutral residues selected from the group consisting of S, T, C, H, Y, N and Q, of one, two or three residues of at least one of the four calmodulin calcium binding sites: site I: residues selected among D20, D22 and D24 residues; site II: residues selected among D56, D58 and N60 residues; site III: residues selected among D93, D95 and N97 residues; site IV: residues selected among D129, D131 and D133 residues; said positions being indicated with reference to the human calmodulin sequence.

Claims29

Dependent claims

A method for the remediation of soil or fluid contaminated with uranium, comprising contacting soil or fluid contaminated with uranium with a reagent comprising a polypeptide, said polypeptide comprising one or more variants of the calmodulin loop of SEQ ID NO:

13, 14, 15 or 16, wherein the calmodulin loop variant comprises the mutation to neutral residues selected from the group consisting of Serine, Threonine, Cysteine, Histidine, Tyrosine, Asparagine and Glutamine of one or more residues selected from the Aspartic acid residues at positions 1 and 3 of SEQ ID NO:
13, SEQ ID NO:
14, SEQ ID NO:
15 and SEQ ID NO:
16 and the Aspartic acid residue at position 5 of SEQ ID NO:
13 and SEQ ID NO:
16, and wherein said calmodulin loop variant is flanked at both ends by an alpha helix.

4 more elements

Dependencies are inferred from the claim text.

Uranium-chelating peptides and uses thereof

Life of this patent

Uranium-chelating peptides and uses thereof

Life of this patent

Abstract

Claims29