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AssigneesOakland University, Vanderbilt University
InventorsXiangqun Zeng, Raymond L. Mernaugh
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An apparatus and methods for binding an analyte of interest in a sample are provided. The apparatus comprises a substrate with an exposed surface with an compound, that is electrostatically charged or capable of forming hydrogen bonds, provided bound to the solid substrate. A recombinant single chain antibody (scFv) molecule specific for the analyte of interest, having one or more amino acids with charged or hydrogen-bond forming sidechains in a linker polypeptide portion, is bound to the layer on the solid substrate. When the analyte of interest is present in the sample the scFv binds the analyte to the solid substrate. The apparatus can be used with an immunoglobulin layer to detect Fc receptors, so as to detect microorganisms such as Staphylococcus aureus having protein A or protein G.
A method of detecting an analyte of interest in a sample, the method comprising:
A method of binding an analyte of interest in a sample, the method comprising:
Dependencies are inferred from the claim text.