US 7,888,123 B2Granted

Method for introducing a gene into labyrinthulomycota

AssigneeFUJIFILM Corporation

InventorsKazuhisa Ono, Tsunehiro Aki, Seiji Kawamoto

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Abstract

An object of the present invention is to provide a transformation system for Labyrinthulomycota that allows the elucidation of biosynthetic mechanisms of lipids such as PUFA and carotenoids as well as for the construction of a high production system and the design and development of novel functional lipid molecules by the control of the mechanisms. The present invention provides a method for introducing a transgene into a cell of Labyrinthulomycota, which comprises introducing into a cell of Labyrinthulomycota a recombinant vector comprising a transgene and a nucleotide sequence which is homologous to a part of chromosomal DNA of Labyrinthulomycota and is capable of homologous recombination with the chromosomal DNA, and then inducing homologous recombination in this homologous nucleotide sequence.

Claims5

Dependent claims

A method for introducing a transgene into chromosomal DNA of a cell of Labyrinthulomycota by homologous recombination comprising,

(a) introducing into a cell of Labyrinthulomycota a recombinant vector comprising at least:
(1) a polynucleotide, wherein the polynucleotide has the polynucleotide sequence set forth as SEQ ID NO:
1, the polynucleotide is homologous to a part of chromosomal DNA in the cell of Labyrinthulomycota, and the polynucleotide undergoes homologous recombination with the part of the chromosomal DNA,
(2) a selection marker gene having a promoter sequence located upstream and a terminator sequence located downstream of the selection marker gene, and
(3) a transgene having a promoter sequence located upstream and a terminator sequence located downstream of the transgene,
(b) selecting a cell of Labyrinthulomycota expressing the selection marker gene.

3 more elements

Dependencies are inferred from the claim text.