Pico liter well holding device and method of making the same

A method of making a holding device for studying cells, comprising the steps of:

• providing a carrier plate;
• applying a first adhesive layer to the carrier plate;
• depositing a curable substrate on the adhesive layer;
• applying a second layer of adhesive to the substrate;
• attaching a wall structure to the second layer of adhesive;
• forming a multiplicity of pico liter wells in the substrate with a template;
• curing the substrate;
• and
• removing the template.

A method of making a holding device, comprising the steps of:

• providing a layer of curable substrate;
• disposing a wall structure on the layer of curable substrate;
• embossing a multiplicity of pico liter wells in a surface of the curable substrate;
• and
• curing the substrate.

A method of making a holding device for studying cells comprising the steps of:

• providing a carrier plate having a plurality of cavities surrounded by walls;
• depositing a curable substrate in each cavity;
• embossing a multiplicity of pico liter wells in the curable substrate with a die;
• and
• curing the substrate.