Superoxide dismutase (SOD) gene and a method of identifying and cloning thereof
AssigneeCouncil of Scientific and Industrial Research
InventorsPardeep Kumar Bhardwaj, Rashita Sahoo, Sanjay Kumar, Paramvir Singh Ahuja
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Abstract
The present invention provides a superoxide dismutase gene from Potentilla atrosanguinea, a construct containing the gene coding for superoxide dismutase and transformed E. coli producing the SOD protein.
Claims5
An isolated Superoxide dismutase (SOD) cDNA of SEQ ID No. 2 obtained from Potentilla atrosanguinea, wherein said cDNA comprises 856 nucleotide bases.
An isolated Superoxide dismutase (SOD) gene of SEQ ID No. 3, wherein said gene comprises 459 nucleotide bases.
An isolated set of primers useful for the amplification of a Superoxide dismutase (SOD) gene of SEQ ID No. 3, wherein
- said Forward primer comprises 5′-ATGGCAAAGGGCGTTGCTGTACTT-3′ (SEQ ID NO:
- 5) and;
- said Reverse primer comprises 5′-TCATCCTTGAAGGCCAATAATACCA-3′ (SEQ ID NO:
- 6).
An expression construct comprising a nucleotide sequence of a superoxide dismutase (SOD) gene of SEQ ID NO 3 which codes for a polypeptide of SEQ ID No. 1 having Superoxide dismutase enzyme activity, a selectable marker and a terminator sequence.
Dependencies are inferred from the claim text.