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US 7,888,087 B2Granted
Fusion protein of Fc-binding domain and calcium-binding photoprotein, gene encoding the same and use thereof
AssigneeChisso Corporation
InventorsSatoshi Inouye, Yuiko Sahara, Junichi Sato
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Abstract
The invention provides a calcium-binding photoprotein which has a high ability to bind to IgG and is capable of more sensitively detecting IgG. A fusion protein of the present invention composed of a ZZ domain having the ability to bind to the Fc region of IgG and the calcium-binding photoprotein apoaequorin is used for the detection and assay of calcium ions and the detection of IgG.
Claims18
A fusion protein comprising:
- (1) a first region selected from the group of:
- (a) a region having the amino acid sequence of SEQ ID NO:
- 1;
- (b) a region having variants of the amino acid sequence of SEQ ID NO:
- 1, wherein said variants have from one to eight deleted, substituted, inserted and/or added amino acids, and which has the ability to bind to the Fc region of IgG;
- and
- (c) a region which has an amino acid sequence that is at least approximately 94% identical to the amino acid sequence of SEQ ID NO:
- 1, and which has the ability to bind to the Fc region of IgG;
- and
- (2) a second region selected from the group of:
- (d) a region having the amino acid sequence of SEQ ID NO:
- 3;
- (e) a region having variants of the amino acid sequence of SEQ ID NO:
- 3, wherein said variants have from one to eight deleted, substituted, inserted and/or added amino acids, and which has the ability to bind to a peroxide of coelenterazine or a peroxide of a coelenterazine derivative so as to form a holoprotein that emits light under the action of a calcium ion;
- and
- (f) a region which has an amino acid sequence that is at least approximately 95% identical to the amino acid sequence of SEQ ID NO:
- 3, and which has the ability to bind to a peroxide of coelenterazine or a peroxide of a coelenterazine derivative so as to form a holoprotein that emits light under the action of a calcium ion,
- wherein the fusion protein has a minimum IgG-detection sensitivity of 1 ng/ml.
15 more elements
A fusion protein comprising:
- (1) a region having a first amino acid sequence of SEQ ID NO:
- 1, and
- (2) a region having a second amino acid sequence of SEQ ID NO:
- 3,
- wherein the fusion protein has a minimum IgG-detection sensitivity of 1 ng/ml.
2 more elements
A fusion protein having the amino acid sequence of SEQ ID NO:
- 5.
Dependencies are inferred from the claim text.