Nε-acyl-L-lysine-specific aminoacylase

An enzyme, wherein the enzyme has the following properties:

• 1) specifically acts on Nε-acyl-L-lysine to catalyze a reaction that releases a carboxylic acid and L-lysine, and also catalyzes the reverse reaction thereof;
• 2) acts on epsilon-amino group of L-lysine;
• 3) has an optimum pH in the range of 8.0 to 9.0 for hydrolysis reaction in Tris-HCl buffer at 37° C. where Nε-acetyl-L-lysine is used as a substrate;
• 4) is stable in the range of pH 6.5 to 10.5 when incubated for 1 hour at 37° C. in Tris-HCl buffer;
• 5) has an optimum reaction temperature of about 55° C. for hydrolysis reaction in Tris-HCl buffer (pH 8.2) were Nε-acetyl-L-lysine is used as a substrate;
• 6) is not inactivated by a treatment at 40° C. for 60 minutes in Tris-HCl buffer (pH 8.2), and retains 75 to 85% residual activity after 60 minutes of treatment at 55° C.;
• 7) is inhibited by o-phenanthroline;
• 8) an activity thereof is increased by cobalt ions;
• 9) a molecular weight thereof is about 60 k as measured by SDS-polyacrylamide electrophoresis;
• 10) amino acid sequence of SEQ ID NO:
• 1 locates at the N-terminal thereof;
• 11) activity on Nα-acetyl-L-Lys is 2.1% or less of the activity on Nε-acetyl-L-Lys;
• and
• 12) is inhibited by L-Cys.