US 7,888,074 B2 · Microchip matrix device for duplicating and characterizing nucleic acids

Abstract

The aim of the invention is to provide a device for duplicating and characterizing nucleic acids almost simultaneously and with a high sample throughput rate. The device consists of a chamber body with a recess whose edge sealingly holds an optically transparent chip. Said chip holds nucleic acids in individual spots on a detection surface. The chamber body is placed on an optically transparent chamber support with a bearing surface, in such a way that a capillary gap, which can be filled with a liquid sample, is formed between the detection surface of the chip facing towards the chamber support and said chamber support. The chamber body is provided with an inlet and an outlet, which are spatially separate from each other, and has a space, which laterally encompasses the chip and which has a gas reservoir. The chamber support is provided with heating elements.

Claims18

Dependent claims

A device for duplicating and characterizing nucleic acids in a reaction chamber, comprising a reaction chamber defined by:

a chamber support having an optically permeable first surface facing the reaction chamber;
a chamber body sealingly and unreleasably placed on the chamber support and including:
a recess having an edge configured to support a chip;
and
an inlet providing fluid communication between the reaction chamber and an environment external to the reaction chamber;
and
a rigid optically permeable chip, sealingly supported by the edge of the recess, and having a second surface facing the reaction chamber, the second surface having an array of multiple different polynucleotide probes immobilized thereon, wherein the first and second surfaces are substantially parallel.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application

10/038,284Filed 2 Jan 2002 · continuation in part

Priority

DE 199 32 4232 Jul 1999

Published

US 2002/0150933 A117 Oct 2002

Granted

15 Feb 2011

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

B01F 33/3031
Micromixers: using electro-hydrodynamic [EHD] or electro-kinetic [EKI] phenomena to mix or move the fluids
B01L 3/5027
With fluid transport, e.g. in multi-compartment structures: by integrated microfluidic structures, i.e. dimensions of channels and chambers are…
B01L 3/508
Containers for the purpose of retaining a material to be analysed, e.g. test tubes: rigid containers without fluid transport within
B01L 2200/142
Process control and prevention of errors: preventing evaporation

Microchip matrix device for duplicating and characterizing nucleic acids

Life of this patent

Microchip matrix device for duplicating and characterizing nucleic acids

Life of this patent

Abstract

Claims18