Method for sequencing nucleic acid molecules
AssigneeGen-Probe Incorporated
InventorDaniel Densham
Life of this patent
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Abstract
The sequence of a target polynucleotide can be determined by: (i) contacting the target polynucleotide with a polymerase enzyme and one of the nucleotides A, T(U), G and C under conditions suitable for the polymerase reaction to proceed; (ii) measuring the time taken for the polymerase to bind to and subsequently dissociate from the target polynucleotide, to thereby determine whether the polymerase has incorporated the nucleotide onto the target polynucleotide; (iii) optionally repeating steps (i) and (ii) with additional nucleotides, to thereby identify the sequence of the target polynucleotide.
Claims23
A method for sequencing a target polynucleotide, comprising:
- (i) contacting the target polynucleotide with a polymerase enzyme and one or amore nucleotides selected from the group consisting of A, T (U), G, and C under conditions suitable for a polymerase reaction to proceed, wherein the contact occurs in a flow cell comprising one or more immobilized polynucleotides;
- (ii) observing the interaction of the polymerase enzyme with the target polynucleotide in the presence of the one or more nucleotides;
- and
- (iii) measuring the time taken for the polymerase to bind to, and subsequently dissociate from, the target polynucleotide to identify whether the polymerase has incorporated a nucleotide onto a nascent polynucleotide paired with the target polynucleotide, and thereby identify the sequence of the target polynucleotide.
Dependencies are inferred from the claim text.