Process and composition for the manufacture of a microbial-based product
AssigneeMicrobios, Inc.
InventorsMatthew Ryan Garner, Joseph Flint
Life of this patent
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Abstract
Processes to produce microorganisms that can be incorporated into a microbial-based product that results in high viable cell yields and shelf-stable products are disclosed. These microbial-based products are useful for inhibiting pathogenic growth and as a food additive. A preferred microorganism is the lactic acid producing bacteria, Lactobacillus amylovorus M35. In one embodiment, the process comprises inoculating a lactobacillus fermentation medium with M35 cells, harvesting the M35 cells at mid to late log phase, concentrating the M35 cells, and preserving the M35 cells at a concentration of at least 5×109 cfu/ml.
Claims9
A method of manufacturing a composition comprising live Lactobacillus acidophilus cells, the method comprising:
- inoculating a bacterial fermentation medium with live Lactobacillus acidophilus cells;
- harvesting the live Lactobacillus acidophilus cells between mid-log and late-log phase of growth wherein a live cell count of Lactobacillus acidophilus cells is at least 1×109 cells/ml;
- concentrating the live Lactobacillus acidophilus cells to a live cell count of at least 5×109 cells/ml;
- and
- preserving the live Lactobacillus acidophilus cells.
A method of manufacturing a composition comprising live Lactobacillus animalis cells, the method comprising:
- inoculating a bacterial fermentation medium with live Lactobacillus animalis cells;
- harvesting the live Lactobacillus animalis cells between mid-log and late-log phase of growth wherein a live cell count of Lactobacillus animalis cells is at least 1×109 cells/ml;
- concentrating the live Lactobacillus animalis cells to a live cell count of at least 5×109 cells/ml;
- and
- preserving the live Lactobacillus animalis cells.
A method of manufacturing a composition comprising live Lactobacillus casei cells, the method comprising:
- inoculating a bacterial fermentation medium with live Lactobacillus casei cells;
- harvesting the live Lactobacillus casei cells between mid-log and late-log phase of growth wherein a live cell count of Lactobacillus casei cells is at least 1×109 cells/ml;
- concentrating the live Lactobacillus casei cells to a live cell count of at least 5×109 cells/ml;
- and
- preserving the live Lactobacillus casei cells.
Dependencies are inferred from the claim text.