Transformed cell with enhanced sensitivity to antifungal compound and use thereof
AssigneeSumitomo Chemical Company, Limited
InventorHiroki Nakajima
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Abstract
The present invention provides a transformed cell in which a polynucleotide having a nucleotide sequence encoding an amino acid sequence of an osmosensing histidine kinase having no transmembrane region is introduced in a functional form into a cell deficient in at least one hybrid-sensor kinase, a method of assaying the antifungal activity of a test substance using the transformed cell, and a method of searching an antifungal compound using the method, and the like.
Claims13
A transformed cell in which a polynucleotide encoding an osmosensing histidine kinase having no transmembrane region is introduced in a functional form into a cell that is deficient in at least one hybrid-sensor kinase, wherein the osmosensing histidine kinase having no transmembrane region has an amino acid sequence homology of 98% or more to the amino acid sequence of SEQ ID NO:
- 55.
An isolated polynucleotide encoding an amino acid sequence selected from the group consisting of:
- (a) an amino acid sequence of an osmosensing histidine kinase having no transmembrane region, which has amino acid sequence homology of 98% or more to the amino acid sequence of any of SEQ ID NO:
- 55 and (b) the amino acid sequence of SEQ ID NO:
- 55.
Dependencies are inferred from the claim text.